| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254001 |
| Chromosome: | chromosome 5 |
| Location: | 1057246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246200 | (1 of 2) PTHR24347//PTHR24347:SF159 - SERINE/THREONINE-PROTEIN KINASE // SERINE/THREONINE-PROTEIN KINASE STK11 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCAGCGGCTTGGGGAATCGCCAGATC |
| Internal bar code: | CAGGTACCGGCCTCTAGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 55.6261 |
| LEAP-Seq n confirming: | 2190 |
| LEAP-Seq n nonconfirming: | 1747 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCATCCTCCTCGTCTG |
| Suggested primer 2: | AGGACCCTTCACATCTGCAC |