| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254016 |
| Chromosome: | chromosome 1 |
| Location: | 2813261 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016651 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATCCAAGCACATCACAGGTTTCCTTGC |
| Internal bar code: | TCCGGTGGGGCCTCCGAACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 98.9437 |
| LEAP-Seq n confirming: | 281 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGTACATAAGCAGGCA |
| Suggested primer 2: | ATGCTGGAGATGTTTCGGTC |