| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254017 |
| Chromosome: | chromosome 12 |
| Location: | 625974 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492250 | TRZ3 | RNase Z-like protein; (1 of 3) K00784 - ribonuclease Z (rnz) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCGCTGGTGCGGCGCCCAACATCGGT |
| Internal bar code: | CTTTTATCTGTCCTGGAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.5684 |
| LEAP-Seq n confirming: | 16609 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGGAAGATAATGCCGTA |
| Suggested primer 2: | TTTACGTGTGGACGCATTGT |