| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254024 |
| Chromosome: | chromosome 7 |
| Location: | 6344060 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357350 | GLB1,PII | Nitrogen regulatory protein PII; (1 of 1) PTHR30115:SF11 - NITROGEN REGULATORY PROTEIN P-II HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGCACTGGGCGGAGACTGCTGACTTG |
| Internal bar code: | TCTTGACTCGACTGTGCAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 87.619 |
| LEAP-Seq n confirming: | 368 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGGTGGCCTCCAGTAAA |
| Suggested primer 2: | GCTGGAAGCTGAGAAGATGG |