| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254037 |
| Chromosome: | chromosome_17 |
| Location: | 6062656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g741200 | EBP1,EB1 | Microtubule plus-end binding protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAATGCATGAATATAGCACCCACCGCACA |
| Internal bar code: | AGGGTGGGCTCGCTGTTATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 99.7191 |
| LEAP-Seq n confirming: | 355 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGCACTCTCTACGCCAT |
| Suggested primer 2: | GAGCAGGAGATCCTGTACGC |