| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254060 |
| Chromosome: | chromosome 2 |
| Location: | 5015812 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104300 | (1 of 3) IPR020835 - Catalase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGCGAATGGAGCCCCTACGATAGAACT |
| Internal bar code: | ACGGAGCCCGATACGGGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 91.6933 |
| LEAP-Seq n confirming: | 574 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCACCAGGACGTTCACC |
| Suggested primer 2: | CAAATTTATGCGTGACGTGG |