| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254071 |
| Chromosome: | chromosome 16 |
| Location: | 6870511 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676309 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCAAGCTCAAAGAGTGAAGGTTCGCTG |
| Internal bar code: | GGCGCGGTGCCATCGCATCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.8733 |
| LEAP-Seq n confirming: | 2364 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTTTTGGGTGTTTGCTGG |
| Suggested primer 2: | CTGTGATGTTCGTTGGGTTG |