| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254076 |
| Chromosome: | chromosome 17 |
| Location: | 3988035 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728850 | RAA15 | component psaA trans-splicing sub complex II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTTTCAATCGCCTAGTTCCCTAGTGC |
| Internal bar code: | TACGTTATTAAATTAAGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.681 |
| LEAP-Seq n confirming: | 625 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTCAATCAAAGAGCCCG |
| Suggested primer 2: | AAACCCTTTCCCTGTTGCTT |