| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254111 |
| Chromosome: | chromosome 5 |
| Location: | 1313329 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244850 | MOG1,NUOB12 | Ran-interacting protein involved in nuclear trafficking | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACACAGTCTTTCAATCGTAGCCGATA |
| Internal bar code: | GCTACGTTGGTCGTCCGCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.4644 |
| LEAP-Seq n confirming: | 1300 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCACGCAATAACTTTCAT |
| Suggested primer 2: | TTACACCGGCAACACGAATA |