| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254125 |
| Chromosome: | chromosome_7 |
| Location: | 753744 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g317850 | FBB10 | Flagellar/basal body protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCGATTGCCTCGGTCTTTGCCAACAGCTC |
| Internal bar code: | CGTTTCTGGTTGTAAACCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 99.7218 |
| LEAP-Seq n confirming: | 717 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCAGACGTAATGCCTTG |
| Suggested primer 2: | CGCGTGTTCAGCTCAAAATA |