| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254138 |
| Chromosome: | chromosome 13 |
| Location: | 3964190 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590900 | APC11 | Anaphase promoting complex subunit 11 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGATAGTTCTTGGGAGTATAACGCTTG |
| Internal bar code: | CGGACTCCTCGGATCTTTGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGCCTACCTCTCCCTAT |
| Suggested primer 2: | TCTTCAAGTAAAGCCGCGAT |