Insertion junction: LMJ.RY0402.254149_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.254149
Chromosome:chromosome 6
Location:4022052
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278204 (1 of 4) PTHR10334//PTHR10334:SF224 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACCCATGTCCGTTTCGCAGTCGTTGCT
Internal bar code:TCGCAAACCGGGCAGTTTCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:99.3814
LEAP-Seq n confirming:2249
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCTACTCATCTGTGCGGACG
Suggested primer 2:GAGACACGGAAGTGTCAGCA