| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254152 |
| Chromosome: | chromosome 7 |
| Location: | 1303293 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322000 | CSD2,NIFS2 | (1 of 1) PTHR11601//PTHR11601:SF20 - CYSTEINE DESULFURYLASE // SELENOCYSTEINE LYASE; Cysteine desulfurase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTCAAACACGAAGGGCGCCATTTCATT |
| Internal bar code: | AGTGGACACTACAGGTGGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 237 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGGTAGTGGAGCGGATG |
| Suggested primer 2: | TTACCAGGCGGTACTTCCAC |