Insertion junction: LMJ.RY0402.254157_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.254157
Chromosome:chromosome 9
Location:4659917
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396698 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGGATCCCTTGTGAGGGACAGGTGTGC
Internal bar code:TCTCACACCAATCAGCTTAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:742
LEAP-Seq percent confirming:98.9498
LEAP-Seq n confirming:3015
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCGACAAACAGGAGGAT
Suggested primer 2:AACCAGACACCGACAGGTTC