Insertion junction: LMJ.RY0402.254158_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.254158
Chromosome:chromosome 11
Location:1172168
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAAAACCCACCTGGATGACGTCTGTGCC
Internal bar code:GGATAGGAAGAACCCGCTAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:66.726
LEAP-Seq n confirming:375
LEAP-Seq n nonconfirming:187
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGTGAGGTTGCGTGTAT
Suggested primer 2:CGGTGTGTGTGTGTGTGTGT