| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254170 |
| Chromosome: | chromosome_16 |
| Location: | 1629358 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g654300 | RSP23 | Radial spoke protein 23 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCCTAAGCTGGTGAACTTTATGACCAGC |
| Internal bar code: | GGTGGTTTCATTAATTGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.5898 |
| LEAP-Seq n confirming: | 6312 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTATAGGCAAAGACGAGG |
| Suggested primer 2: | CACTATGCAGCTCTGGGTCA |