| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254170 |
| Chromosome: | chromosome 16 |
| Location: | 6270062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674850 | (1 of 1) PTHR14625//PTHR14625:SF3 - MICROCEPHALIN // MICROCEPHALIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCCATTTCAGGAGATCAAGTACCTGGA |
| Internal bar code: | CGACGTGCAGGGCGACCGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 98.6934 |
| LEAP-Seq n confirming: | 1133 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAAGGCCAGGAAGTCAG |
| Suggested primer 2: | GGCACGAAGGAGAAGTTCAG |