| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254185 |
| Chromosome: | chromosome 11 |
| Location: | 1899900 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468100 | PDE30 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACGCTGTACGACGGAATGACGGCCGCG |
| Internal bar code: | GGCGCGTCCCTCACAACAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 98.7172 |
| LEAP-Seq n confirming: | 6695 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCGTTGGTCTGAGTGTG |
| Suggested primer 2: | CAAGTTCGGTAACACTGCGA |