| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254194 |
| Chromosome: | chromosome 12 |
| Location: | 1692944 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499301 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGGCCGCGGGTAGCTAGGTGCAAGGGC |
| Internal bar code: | GCGTAAGTTGGGGCAGGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.7416 |
| LEAP-Seq n confirming: | 386 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTATTGACGAGTGCCTG |
| Suggested primer 2: | GTGAATCTTGCTGTGCCTCA |