| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254206 |
| Chromosome: | chromosome 2 |
| Location: | 3329617 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095091 | (1 of 1) PTHR10108//PTHR10108:SF795 - METHYLTRANSFERASE // CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCCCCAACTCTTGCCAACTGGCAGCGT |
| Internal bar code: | GGTGTGTGGGGTCGTTGATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 98.8669 |
| LEAP-Seq n confirming: | 349 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACAGTAACTCCGGCAT |
| Suggested primer 2: | AGGACGGACTGCAATCAAAC |