| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254220 |
| Chromosome: | chromosome 16 |
| Location: | 1978769 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656500 | ALA3 | (1 of 1) K01530 - phospholipid-translocating ATPase (E3.6.3.1); ATPase, phospholipid transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGATTGCGCGACGGCAAGCCACCGCACA |
| Internal bar code: | CATCGCGGTCTAGCGTGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.4899 |
| LEAP-Seq n confirming: | 8386 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCACCACCGTAGTTTTT |
| Suggested primer 2: | TCTTCCTGCCCATGTACTCC |