| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254234 |
| Chromosome: | chromosome 2 |
| Location: | 5421455 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107450 | ATS2 | ATP-sulfurylase; (1 of 2) 2.7.7.4 - Sulfate adenylyltransferase / Sulfurylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATGGATGAGAGGTGCAGGCAGCGCGG |
| Internal bar code: | GCAACGCCGGTGTGCTTACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 98.4615 |
| LEAP-Seq n confirming: | 320 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCCTCACGCTCTTCTA |
| Suggested primer 2: | ATGGGTACAGCGGTAGATCG |