| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254243 |
| Chromosome: | chromosome 14 |
| Location: | 1483029 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACATACATATACGGTCCCCGTGTACCTG |
| Internal bar code: | AACGCAATCAAGGTGCAAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 99.3016 |
| LEAP-Seq n confirming: | 1564 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCATGTGGATATGGAGAA |
| Suggested primer 2: | TGCACCCTACGGGACTTTAC |