Insertion junction: LMJ.RY0402.254254_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.254254
Chromosome:chromosome 7
Location:6048016
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g355050 (1 of 1) IPR003610 - Carbohydrate-binding module family 5/12 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAGATTGCCGCCCAGGTGTCCAAGTCGC
Internal bar code:AAGGTCTGGCGGGCGTGATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:592
LEAP-Seq percent confirming:98.1395
LEAP-Seq n confirming:211
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTAATGGTGGCAACATGG
Suggested primer 2:AACGATTCGTCCGTACAAGG