| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254255 |
| Chromosome: | chromosome 16 |
| Location: | 559872 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692500 | (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAATGGTGCACGTATGACCCTGCCGCTA |
| Internal bar code: | AACCGAGTGAGTGCTTGAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.1987 |
| LEAP-Seq n confirming: | 619 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGACTGCATACGGTACA |
| Suggested primer 2: | ACTTAGCGTTGAGCGCTTTC |