| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254264 |
| Chromosome: | chromosome 10 |
| Location: | 1770372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430800 | (1 of 3) PTHR10566//PTHR10566:SF76 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // PROTEIN Y32H12A.7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCCTCCCCCTTCCTCTCGCTCCCTCCC |
| Internal bar code: | CCGTAGACTCCGCATCAGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGGGTTTCTTCAAGGCG |
| Suggested primer 2: | AACTTTGCACCTGAAATCGG |