| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254277 |
| Chromosome: | chromosome 2 |
| Location: | 7377769 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144850 | CGLD26b,CGLD26 | Conserved in the Green Lineage and Diatoms | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAACCACAGGACACTCAGCCGACGCAGT |
| Internal bar code: | CAGTTCGTCCGTTCCCAACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 139 |
| LEAP-Seq percent confirming: | 88.1455 |
| LEAP-Seq n confirming: | 751 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATAGTTGTCGACCGCCG |
| Suggested primer 2: | AGCGCCACATACCCAGTTAC |