| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254281 |
| Chromosome: | chromosome 1 |
| Location: | 4870085 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033700 | (1 of 1) PF01933 - Uncharacterised protein family UPF0052 (UPF0052) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGAAGGCGTAGCCAGGTACAAGTGGG |
| Internal bar code: | GGTAATATCCATCTAGAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 99.1228 |
| LEAP-Seq n confirming: | 226 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATCACCTACCCACCCCT |
| Suggested primer 2: | TACACACCGTTCGCATGAAT |