| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254297 |
| Chromosome: | chromosome 6 |
| Location: | 6786672 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294950 | ENR1 | (1 of 1) 1.3.1.9 - Enoyl-[acyl-carrier-protein] reductase (NADH) / NADH-specific enoyl-ACP reductase; Enoyl-ACP reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACATCGACCCTTGTAGGCGTTACTCAA |
| Internal bar code: | CTAAGGTGCTTCCACCCACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 9 |
| LEAP-Seq percent confirming: | 99.6815 |
| LEAP-Seq n confirming: | 313 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACCCAAACCTATGCTCG |
| Suggested primer 2: | ACCTACCAGCAGCTCCAAGA |