| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254301 |
| Chromosome: | chromosome 9 |
| Location: | 5800077 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402108 | (1 of 1) PTHR13167//PTHR13167:SF25 - UNCHARACTERIZED // PIEZO, ISOFORM E; Piezo R-Ras-binding domain membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCCAGCTCGTGCACACAGCAAACACCG |
| Internal bar code: | CCCGACGGAACAATGGAAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 92.1668 |
| LEAP-Seq n confirming: | 906 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCAACACTCTTATCGCC |
| Suggested primer 2: | GACCAGCACTGTTGCTTTCA |