| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254311 |
| Chromosome: | chromosome 12 |
| Location: | 4823349 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524600 | (1 of 1) PTHR17630//PTHR17630:SF50 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTCTCTTCATGCGCAGGGACTAGGCCC |
| Internal bar code: | GCTTCTTCGACGGCCTTCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 52.6005 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 401 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACATCTCCATTGGGACT |
| Suggested primer 2: | AGGCTTATGCCTCATGCAGT |