Insertion junction: LMJ.RY0402.254316_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.254316
Chromosome:chromosome 17
Location:4946789
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g734612 MRP2 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; ABC transporter, multidrug resistance associated protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTGGGATGGATCCTGTCCACCATGTTC
Internal bar code:AGGGGGAACCCGGCTAGACCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:501
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:105
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGTACAAAGCGTGTGGTC
Suggested primer 2:AACGTGTTGAACAGCGACAG