Insertion junction: LMJ.RY0402.254317_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.254317
Chromosome:chromosome 17
Location:871282
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g702500 TAB2 (1 of 1) PTHR34556//PTHR34556:SF2 - FAMILY NOT NAMED // F17A17.35 PROTEIN; conserved chloroplast PsaB RNA binding protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTTTGTGCAGTGGGTCCTGCCGAGGGG
Internal bar code:GGGGTGGTCCCCCACGGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:174
LEAP-Seq percent confirming:98.7342
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATCGACTTCTGTTCTCGCC
Suggested primer 2:CCTGAAGTGCCTAAAGACGC