Insertion junction: LMJ.RY0402.254329_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.254329
Chromosome:chromosome 1
Location:4604857
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g031650 CGLD12 Conserved in the Green Lineage and Diatoms; (1 of 2) PF05637 - galactosyl transferase GMA12/MNN10 family (Glyco_transf_34) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCAACGTAGGCGCAGCGTAGCGGCACTC
Internal bar code:GCGTCAGGCAGTACAGGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTGATGCTTCCACTTGA
Suggested primer 2:AAGGGACATCACTAATGCCG