| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254381 |
| Chromosome: | chromosome 9 |
| Location: | 6147201 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405550 | TIM21 | Mitochondrial inner membrane translocase subunit; (1 of 1) K17796 - mitochondrial import inner membrane translocase subunit TIM21 (TIM21) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCGTGTGGCTTTCGCGGTTGAGCGTC |
| Internal bar code: | AGTGCACTTGGCGCATACACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.6083 |
| LEAP-Seq n confirming: | 6103 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | CACTAGGGTCGGCAACAAAT |