| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254400 |
| Chromosome: | chromosome 3 |
| Location: | 3129128 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165000 | EFG10,LPA1 | (1 of 1) K03596 - GTP-binding protein LepA (lepA); Translation elongation factor EFG/EF2, LepA-related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCTGTGGTAGTCATATGCGTGGAGCGC |
| Internal bar code: | GGTGCCGGCAGACGGTGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 19.0476 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACTGCGCTACTGTACCT |
| Suggested primer 2: | ACCTGTTGACGGTGAACTCC |