| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254401 |
| Chromosome: | chromosome 6 |
| Location: | 3342026 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGGGGAGGCGGAATGGGGACACGAAAG |
| Internal bar code: | CTGCGTTCGGCCCTGCTTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 87 |
| LEAP-Seq percent confirming: | 99.7588 |
| LEAP-Seq n confirming: | 4964 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACTACCTTCACGGCCAC |
| Suggested primer 2: | CTTATCGCCTTGCTGTTGCT |