| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254416 |
| Chromosome: | chromosome 16 |
| Location: | 5374428 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681600 | CSV14 | Chlamydomonas-specific family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACACCGCCTGCGACACCTAGCCGGGAC |
| Internal bar code: | ACGCGGGGCTCCCGCCAATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCAGAGTTGCGTTTTG |
| Suggested primer 2: | GCTGCTGGGTTGTTATTGGT |