| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254417 |
| Chromosome: | chromosome 3 |
| Location: | 4266589 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174400 | CDO1 | Cysteine dioxygenase, regulated by copper nutrition; (1 of 2) 1.13.11.20 - Cysteine dioxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATTATACATCCTCGCTCACAGTTGTCCC |
| Internal bar code: | GCGGCCCCCATCCCGCCCCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.5462 |
| LEAP-Seq n confirming: | 1755 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGCACAGCATTGTACGG |
| Suggested primer 2: | GGAATGCGGTTGTTCTTTGT |