Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.254456 |
Chromosome: | chromosome_6 |
Location: | 6372428 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g292150 | sense | 3'UTR|outside_mRNA | ||
Cre06.g292183 | CAM13 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGACGCGGCCAATGTGCAACAGCCTCAAT |
Internal bar code: | ACGGCCACTGCGCGGTGGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 410 |
LEAP-Seq percent confirming: | 94.4664 |
LEAP-Seq n confirming: | 3585 |
LEAP-Seq n nonconfirming: | 210 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGGCCGGTATGTTCATCTG |
Suggested primer 2: | GGTTTTGGGACTGCACTGTT |