| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254466 |
| Chromosome: | chromosome 5 |
| Location: | 2882483 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGCAGGCGCACGGGACACACCAGCCGG |
| Internal bar code: | CCCAGGAGGCGAATCCCAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 656 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTGTGTACAGACGGGCT |
| Suggested primer 2: | AGCTGAACGCCAAGAAAGAG |