| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254474 |
| Chromosome: | chromosome 11 |
| Location: | 1423322 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467726 | (1 of 1) K06316 - oligosaccharide translocation protein RFT1 (RFT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCGGCCAGACACCCGGCCACGCCGATG |
| Internal bar code: | CAGCCGTCTTGAAACGAAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 99.0654 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCAAACATCCAACACCG |
| Suggested primer 2: | GTTGCACATTGTTTGCCATC |