| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254507 |
| Chromosome: | chromosome 11 |
| Location: | 1894760 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468100 | PDE30 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTGTGTGTATATGTTGCAAACTGCAAA |
| Internal bar code: | AGGCGTGCCAAAGGAGGCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 93.4524 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTACCGGCAACCTTACG |
| Suggested primer 2: | CTTCTTTCTACGTACGCGCC |