Insertion junction: LMJ.RY0402.254508_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.254508
Chromosome:chromosome 3
Location:4890784
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g179450 SRR7 (1 of 1) K09624 - protease, serine, 12 (neurotrypsin, motopsin) [EC:3.4.21.-] (PRSS12); Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCTTCAAAGCGGAGGGGAGAGAAGCGAG
Internal bar code:TAGGGGGCGAGTAGCTACAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:170
LEAP-Seq percent confirming:99.6324
LEAP-Seq n confirming:271
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGCAGGAGTAGAAGAGTG
Suggested primer 2:TACTACAGGGGACAGTGGGG