| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254527 |
| Chromosome: | chromosome 1 |
| Location: | 575342 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003376 | (1 of 1) K09522 - DnaJ homolog subfamily C member 2 (DNAJC2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTGACATGTAGCCCTATGGTATGTAGT |
| Internal bar code: | CACTGATCTGTCAACGTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 98.9789 |
| LEAP-Seq n confirming: | 6010 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCTTCTCTCCTCGCTCG |
| Suggested primer 2: | GTGAGCTTCTCCACCTCGTC |