Insertion junction: LMJ.RY0402.254545_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.254545
Chromosome:chromosome 17
Location:1140220
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g704300 FAP47 Flagellar Associated Protein 47; (1 of 1) PTHR23053//PTHR23053:SF2 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // PRIMARY CILIARY DYSKINESIA PROTEIN 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCGCTTGAGCGGCGCGCGCGGGTTGACC
Internal bar code:GGGAACGTGCACTCTGTGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCGCTGTTGATTGGAGT
Suggested primer 2:GCGCATTACCAACTTCGATT