Insertion junction: LMJ.RY0402.254546_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.254546
Chromosome:chromosome 3
Location:7700348
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g203700 DEGO1,DEG12 DegP-type protease-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCTTCCGCATGAACACGCCATGCCTAGG
Internal bar code:TGGTTATGAGGGGAAAGTCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:618
LEAP-Seq percent confirming:99.0709
LEAP-Seq n confirming:1173
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCGCCATGGTATCGTCT
Suggested primer 2:TCCTTGACCCATTGTCTTCC