Insertion junction: LMJ.RY0402.254553_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.254553
Chromosome:chromosome 3
Location:4683105
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g177650 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGGCCTTGTCCACCAGCGGCGGCGGCA
Internal bar code:CACTGCCGAGAAGGGCCGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:708
LEAP-Seq percent confirming:94.6565
LEAP-Seq n confirming:1984
LEAP-Seq n nonconfirming:112
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGCTACTGATCTCCCTG
Suggested primer 2:CCACACACACACACACTCCA