| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254563 |
| Chromosome: | chromosome 3 |
| Location: | 3195652 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165400 | COX16 | Cytochrome c oxidase assembly factor | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGTCGCGTACAATGCGCTAACATAAAC |
| Internal bar code: | GAGTTGGATTGTTCTCCAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 96.3362 |
| LEAP-Seq n confirming: | 7599 |
| LEAP-Seq n nonconfirming: | 289 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACCCTAGAAAGGGCCAC |
| Suggested primer 2: | AAACGGAAGGACACTGATGG |