Insertion junction: LMJ.RY0402.254601_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.254601
Chromosome:chromosome 4
Location:1102933
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g216150 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTTTGTTGGCGGCGCACCCGTGACTGAC
Internal bar code:GTTGGAGCCCATTTGTGTCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGGCTGTTCAATACACG
Suggested primer 2:AAGGTGCGGTTTCTAAGGGT